×

HOW TO GET HELP

1 Login or create new account.
2 Review your contact information.
3 Request & FREE Quote

If you still have problems, please let us know, by sending an email to [email protected] . Thank you!

OFFICE HOURS

Mon-Fri 9:00AM - 5:00PM
Sat - 9:00AM-3:00PM
Sundays by appointment only!
QUESTIONS? CALL DR. HEATHER RAY 306.292.5562
  • SUPPORT

Rochfort Gene Consulting

Rochfort Gene Consulting

Plant Genetics and Genomics Consultant

T (306) 292.5562
Email: [email protected]

Rochfort Gene Consulting
134 107th Street West. Saskatoon. SK S7N1P1 Canada

Open in Google Maps
  • HOME
  • CORPORATE
  • SERVICES
  • EXPERTISE
  • PROJECTS
  • PARTNERS
  • AG-HUB
  • CONTACT
FREEQUOTE
  • Home
  • Privacy Policy
  • Publication
  • Bioassay For Assessing Resistance Of Arabidopsis Thaliana L.
April 14, 2021

Bioassay For Assessing Resistance Of Arabidopsis Thaliana L.

Bioassay For Assessing Resistance Of Arabidopsis Thaliana L.

by Rochfort Gene Consulting / Saturday, 01 January 2005 / Published in Publication, Research

Bioassay for assessing resistance of Arabidopsis thaliana L. (Heynh.) to the adult crucifer flea beetle, Phyllotreta cruciferae (Goeze) (Coleoptera: Chrysomelidae)

Abstract
A bioassay arena and a laboratory screening protocol were developed for assessing lines of Arabidopsis thaliana L. (Heynh.) for feeding damage by the adult crucifer flea beetle, Phyllotreta cruciferae (Goeze). The arena consists of a 96-well microtitre plate with a modified top to contain flea beetles and allow ventilation. Eight lines of A. thaliana, arranged in an 8 x 8 Latin square design, were screened simultaneously in each arena using 50 starved flea beetles. Two cotyledons and the first pair of true leaves per plant were rated visually under a dissecting microscope using a visual damage rating scale. The protocol was used to screen 29 wild ecotypes, eight mutant lines and a single transgenic line of A. thaliana. Discrimination between both cotyledon and leaf tissue was apparent for young beetles that were both non-reproductive or reproductive, but not for old reproductive beetles. Differences were observed between Asian and European ecotypes of A. thaliana, suggesting that geographic origin may play a role in susceptibility of Arabidopsis ecotypes to flea beetle feeding. The transparent testa regulatory gene mutants (lines 82, 111, 164) were most susceptible to flea beetle feeding, possibly indicating a role for anthocyanins and/or flavonoids in governing flea beetle susceptibility. Significant variation in damage levels indicates that expression of flea beetle resistance in the Arabidopsis genome is plastic, and that potential exists to use the wide array of publicly available Arabidopsis germplasm as tools in the transfer of resistance to agronomically important host plants.

Article Contributors:

Rebecca H Hallett at University of Guelph
Heather Ray at Rochfort Gene Consulting
Juliana Soroka
at Agriculture and Agri-Food Canada
Jennifer Holowachuk
Margaret Y. Gruber

Bioassay for assessing resistance of Arabidopsis thaliana L. (Heynh.) to the… | Request PDF. Available from: https://www.researchgate.net/publication/269949807_Bioassay_for_assessing_resistance_of_Arabidopsis_thaliana_L_Heynh_to_the_adult_crucifer_flea_beetle_Phyllotreta_cruciferae_Goeze_Coleoptera_Chrysomelidae

Tagged under: Canadian Journal of Plant Science

About Rochfort Gene Consulting

What you can read next

Expression And Disease Response
Reduce Raffinose Oligosaccharide Content in Pea Seeds
Forage Crops

You must be logged in to post a comment.

Recent Posts

  • Genome Editing of Crops

    A Renewed Opportunity for Food Security Abstrac...
  • Faba Bean

    Transcriptome Analysis from Etiolated Seedling ...
  • Mineral Micronutrient Content in Saskatchewan, Canada

    Mineral Micronutrient Content of Cultivars of F...
  • Metabolomic Shifts In Brassica Napus Lines

    Metabolomic shifts in Brassica napus lines with...
  • Reduce Raffinose Oligosaccharide Content in Pea Seeds

    Over-expression of α-galactosidase in pea seeds...

Recent Comments

    Archives

    • January 2017
    • March 2015
    • July 2014
    • May 2014
    • December 2013
    • March 2010
    • September 2009
    • June 2009
    • October 2006
    • January 2005
    • October 2003
    • August 2003
    • January 2001
    • January 1999
    • August 1998
    • July 1992
    • August 1987

    Categories

    • Ag-hub
    • Publication
    • Research

    Meta

    • Log in
    • Entries feed
    • Comments feed
    • WordPress.org

    Featured Posts

    • Genome Editing of Crops

      0 comments
    • Faba Bean

      0 comments
    • Mineral Micronutrient Content in Saskatchewan, Canada

      0 comments
    • Metabolomic Shifts In Brassica Napus Lines

      0 comments
    • Reduce Raffinose Oligosaccharide Content in Pea Seeds

      0 comments
    • PRIVACY POLICY
    • TERMS OF SERVICE
    • COPYRIGHT
    Rochfort Gene Consulting

    © 2019 All rights reserved. Rochfort Gene Consulting | Dr. Heather Ray
    Saskatoon, Saskatchewan. Canada

    TOP