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  • Cloned Tetracycline Resistance
April 14, 2021

Cloned Tetracycline Resistance

Cloned Tetracycline Resistance

by Rochfort Gene Consulting / Saturday, 01 August 1987 / Published in Ag-hub, Publication

Characterization and expression of a cloned tetracycline resistance determinant from Campylobacter jejuni plasmid pUA466

Abstract
A tetracycline resistance (Tcr) determinant previously cloned from the Campylobacter jejuni plasmid pUA466 (D. E. Taylor, J. Bacteriol. 165:1037-1039, 1986) was localized by restriction endonuclease mapping, subcloning, and Tn1000 insertion mutagenesis to a 2-kilobase region consisting of 1.8- and 0.2-kilobase HincII fragments. Tcr encoded by the cloned fragment (pUOA1) was expressed constitutively in Escherichia coli. A protein with an apparent molecular weight of 68,000 encoded by pUOA1 was produced in an in vitro transcription-translation system and in minicells. Tn1000 insertions which resulted in inactivation of Tcr expression also resulted in an alteration in the 68,000-molecular-weight protein. Some mutants specified a truncated protein, whereas others completely lost the ability to specify the protein. The protein which appears to be involved in the expression of Tcr specified by C. jejuni plasmids is of approximately the same molecular weight as the protein specified by the streptococcal class M determinant. This finding is consistent with our previous results which indicate that homology exists between the Tcr determinant from C. jejuni and a 5-kilobase HincII probe derived from the streptococcal class M determinant.

Article Contributors:

D E Taylor
Heather Ray
at Rochfort Gene Consulting
Koji Hiratsuka

Elias Manavathu at Medical College of Georgia/Augusta University

Characterization and expression of a cloned tetracycline resistance determinant from Campylobacter jejuni plasmid pUA466 (PDF Download Available). Available from: https://www.researchgate.net/publication/20119046_Characterization_and_expression_of_a_cloned_tetracycline_resistance_determinant_from_Campylobacter_jejuni_plasmid_pUA466

Tagged under: Journal of Bacteriology

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