Genome Editing of Crops
A Renewed Opportunity for Food Security Abstract Genome editing of crop plants is a rapidly advancing technology whereby targeted mutations can be introduced into a plant genome in a highly specific manner and with great precision. For the most part, the technology does not incorporate transgenic modifications and is far superior to conventional chemical mutagenesis.
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Reduce Raffinose Oligosaccharide Content in Pea Seeds
Over-expression of α-galactosidase in pea seeds to reduce raffinose oligosaccharide content Abstract The raffinose family of oligosaccharides (RFO) is a series of complex carbohydrates stored in seeds of many plant families, especially in legumes. The digestive system of nonruminant animals, including that of humans, cannot break down all of the chemical bonds in these carbohydrates;
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In Vitro Ruminal Digestion Of Anthocyanidin
In vitro ruminal digestion of anthocyanidin-containing alfalfa transformed with the maize Lc regulatory gene Abstract In vitro ruminal digestion was conducted on novel “purple-green” alfalfa forage that accumulated anthocyanidin and proanthocyanidins through the expression of a maize bHLH anthocyanidin regulatory gene (Lc). The Lc-transgenic genotypes were compared with parental (non-transformed, NT) alfalfa in their in
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Bioassay For Assessing Resistance Of Arabidopsis Thaliana L.
Bioassay for assessing resistance of Arabidopsis thaliana L. (Heynh.) to the adult crucifer flea beetle, Phyllotreta cruciferae (Goeze) (Coleoptera: Chrysomelidae) Abstract A bioassay arena and a laboratory screening protocol were developed for assessing lines of Arabidopsis thaliana L. (Heynh.) for feeding damage by the adult crucifer flea beetle, Phyllotreta cruciferae (Goeze). The arena consists of
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Proanthocyanidin Biochemistry And Molecular Regulation
New perspectives on proanthocyanidin biochemistry and molecular regulation Abstract Our understanding of proanthocyanidin (syn. condensed tannin) synthesis has been recently extended by substantial developments concerning both structural and regulatory genes. A gene encoding leucoanthocyanidin reductase has been obtained from the tropical forage, Desmodium uncinatum, with the latter enzyme catalyzing formation of (+)-catechin. The BANYULS gene
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Fusarium Sambucinum
Responses of potato tuber to infection by Fusarium sambucinum Abstract Fusarium sambucinumcauses dry rot in tubers ofSolanum tuberosum. Since relatively little is known about the nature of dry rot disease development, infection ofF. sambucinumon tissue discs prepared from tubers of potato varieties that are susceptible or moderately resistant to this disease was studied. Increases in
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Expression And Disease Response
Transformation of potato with cucumber peroxidase: Expression and disease response Abstract Peroxidase is thought to be important in a variety of plant defence responses against pathogens. Potato was transformed with a cucumber anionic peroxidase under the control of the cauliflower mosaic virus 35S promoter, which is highly expressed in potato tuber tissue. This particular peroxidase
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Allelopathic Chemicals
Variation within flax (Linum usitatissimum) and barley (Hordeum vulgare) in response to allelopathic chemicals Abstract A possible method of manipulating allelopathy would be to develop crop varieties showing an increased tolerance to allelopathic chemicals. We therefore examined four flax (Linum usitatissimum) varieties and two wild Linum species in the presence of p-coumaric acid and four
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