Transcriptome Analysis from Etiolated Seedling and Developing Seed Coat of Key Cultivars for Synthesis of Proanthocyanidins, Phytate, Raffinose Family Oligosaccharides, Vicine, and Convicine
Faba bean (Vicia faba L.) has been little examined from a genetic or genomic perspective despite its status as an established food and forage crop with some key pharmaceutical factors such as vicine and convicine (VC), which provoke severe haemolysis in genetically susceptible humans. We developed next-generation sequencing libraries to maximize information to elucidate the VC pathway or relevant markers as well as other genes of interest for the species. One selected cultivar, A01155, lacks synthesis of the favism-provoking factors, VC, and is low in tannin, while two cul-tivars, SSNS-1 and CDC Fatima, are wild-type for these factors. Tissues (5-to 6-d-old root and etiolated shoot and developing seed coat) were selected to maximize the utility and breadth of the gene expression profile.
Heather Ray at Rochfort Gene Consulting
Cheryl Ann Bock at National Research Council Canada
Fawzy Georges at Jene Quests Corporation
Faba Bean: Transcriptome Analysis from Etiolated Seedling and Developing Seed Coat of Key Cultivars for Synthesis of Proanthocyanidins, Phytate, Raffinose Family Oligosaccharides, Vicine, and Convicine (PDF Download Available). Available from: https://www.researchgate.net/publication/274710546_Faba_Bean_Transcriptome_Analysis_from_Etiolated_Seedling_and_Developing_Seed_Coat_of_Key_Cultivars_for_Synthesis_of_Proanthocyanidins_Phytate_Raffinose_Family_Oligosaccharides_Vicine_and_Convicine
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